Isolation and characterization of MDR- Klebsiella pneumonia phage from Euphrates River in Al-Anbar city

Abstract

In Iraq, there is no active action for Al-Ramadi teaching hospital for maternity and children waste which is straightforwardly discharge in to Euphrates River in Al-Anbar city. As a result, the numbers of multi-drugs resistant bacteria were raised specially in Enterobacteriaceae family (Klebsiella pneumonia). Nevertheless, modern studies are using phages isolated from similar ecosystem as a biologic alternate for antimicrobic remedy. The present work has an aim to isolate Klebsiella pneumonia and categorise its antibiotic resistance elements from Euphrates River in Al-Anbar city, Iraq. As well as isolation of phages from the similar collected river water specimens then examining their repressive consequence on MDR- Klebsiella pneumonia isolates. Water specimens were taken from 5 sites in to Euphrates River near to the residues of sewage estuaries that run straight to the main river for many regions of Ramadi city. These specimens were inoculated on MacConkey agar plates for morphologic appearance, and biochemical characters by API- 20E test and confirmed by VITEK 2. Disc diffusion technique was performed to identify antibiotic sensitivity.