Effect of berberine in comparison to metformin on the biophysical and biochemical parameters in diabetic albino Wistar rats

Abstract

Introduction: Diabetic endothelial dysfunction is accompanied by increased oxidative stress and upregulated proinflammatory and inflammatory mediators in the endothelial vasculature. Aim of this study is to investigate the effect of Berberine, a natural alkaloid, on the oxidative stress, inflammation and its anti-oxidant effect in streptozotocin diabetic rats and to compare the effectiveness of FF with that of Metformin (Met) Material & Methods: This experimental animal study was conducted at animal house. The sample size included 174 albino wistar rats divided into 3 Groups, one control groups (C) Diabetic and untreated and two test groups. T1 Diabetic and treated with metformin 75 mg/kgwt/day) and T2 (T – Diabetics treated with Berberine(Ber) 100 mg/kgwt/day), with 58 rats in each group (29 male & 29 female). All the rats were treated with streptozotocin intra peritoneally and the diabetic state was induced.  T1 group was treated with metformin 75 mg/kg/wt/day. The T2 group of rats were treated with Berberine at a dose of 100 mg/kgwt/day. Blood sample was drawn from retro orbital plexus of animals and the biophysical and biochemical parameters were tested at an interval of 3, 6 and 12-months duration.