Comparative analysis of the oral opportunistic microorganism growth on the surface of collagen membranes: experimental study

Abstract

Relevance. Nowadays, in oral surgery, it is still relevant to increase the effectiveness of collagen membranes in the oral cavity in the condition of potential microbial contamination of the wound.Purpose. The study aimed to conduct a comparative analysis of the growth of opportunistic oral bacteria on various collagen membranes.Material and methods. Based on the I.I. Mechnikov Research Institute for Vaccines and Sera, the study conducted a comparative analysis of the growth of opportunistic oral bacteria on the surface of resorbable collagen membranes developed by the Institute of Regenerative Medicine of the Sechenov University and an already existing foreign alternative. Microbial growth was assessed for S. aureus, S. mutans and C. albicans as the most significant microorganisms isolated from the oral cavity. The quantitative composition of microorganisms was analyzed in the control group without membranes and in groups with membranes (Group 2 – customized collagen membrane 2, Group 3 – membrane 3 with recombinant human lactoferrin, Group 4 – Mucograft membrane) on days 3, 5, and 7 from the start of membrane surface colonization. In all groups, we also evaluated the pH change in a 10% sucrose solution under the influence of biofilm microorganisms. Statistical data processing included the calculation of mean, standard deviations and medians, and comparison between groups was carried out using the Kruskal-Wallis test after assessing the samples' distribution normality. The results were statistically significant at a minimum of 95%.Results. The performed analysis showed statistically significant differences in the quantitative composition of the microbial biofilm on the surface of all three membranes. The developed collagen membrane with lactoferrin showed the best result at all times, while the customized collagen membrane did not significantly differ from the Mucograft membrane in CFU values and medium pH change. This provision was statistically significant for all three studied microorganisms.Conclusion. The study showed the potential of using manufactured collagen membranes to eliminate soft tissue defects in the oral cavity under the condition of their surfaces' possible contamination as an alternative to foreign membranes.