Metagenomics combined with activity-based proteomics point to gut bacterial enzymes that reactivate mycophenolate

Author:

Simpson Joshua B.1,Sekela Josh J.1,Graboski Amanda L.2,Borlandelli Valentina B.3,Bivins Marissa M.2,Barker Natalie K.4,Sorgen Alicia A.5,Mordant Angie L.4,Johnson Rebecca L.6,Bhatt Aadra P.7,Fodor Anthony A.8,Herring Laura E.4,Overkleeft Hermen3,Lee John R.9,Redinbo Matthew. R.110ORCID

Affiliation:

1. Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

2. Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

3. Department of Bioorganic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

4. UNC Proteomics Core Facility, Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

5. Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, USA

6. Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

7. Division of Gastroenterology and Hepatology, Department of Medicine, Center for Gastrointestinal Biology and Disease, and the Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

8. Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC, USA

9. Department of Medicine, Division of Nephrology and Hypertension, New York, New York, USA

10. Department of Biochemistry and Biophysics, Department of Microbiology and Immunology, and the Institute for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

Funder

NIH

K Awards at NIDDK

NSF

Career Development Award from the Crohn’s and Colitis Foundation

CGIBD

NORC

Cancer Center Core Support Grant

Publisher

Informa UK Limited

Subject

Infectious Diseases,Microbiology (medical),Gastroenterology,Microbiology

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