Histochemical Characterization of Renal Phosphatases in Rats Treated With Lead

Abstract

Seven groups of 84 male white rats ( Rattus norvegicus) were exposed to lead acetate (0, 0.125, 0.25, 0.5, 1.0, 2.0, and 4% for 1 to 5 months) in drinking water to investigate possible histochemical changes in the activity of renal phosphatases due to lead intoxication. In comparison with respective control rats, chronic exposure to subtoxic doses of lead increased the activities of acid-, neutral-, alkaline-, and membrane-bound Na+-K+ adenosine triphosphatase and glucose-6-phosphatase. In contrast, significant reduction in the activities of 5-nucleotidase and mitochondrial adenosine triphosphatase was observed. Changes in the activity of renal phosphatases were seen mainly in the epithelium of the proximal tubules and, to a lesser extent, in the medullary interstitial cells and the glomeruli as well. These histochemical findings led us to conclude that such changes in the activity of phosphatases were due to long-term exposure to subtoxic doses and could be an adaptation to the metabolic, structural, and functional alterations in the organelles of the renal cells due to lead intoxication.