FRET sensor-based quantification of intracellular trehalose in mammalian cells-Reference-Cited by-同舟云学术

FRET sensor-based quantification of intracellular trehalose in mammalian cells

Published:2016-01-02 Issue:1 Volume:80 Page:162-165
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kikuta Shingo¹²,Hou Bi-Huei³,Sato Ryoichi²,Frommer Wolf B³,Kikawada Takahiro¹

Affiliation:

1. Insect Mimetics Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan

2. Graduate School of Bio-Applications & Systems Engineering (BASE), Tokyo University of Agriculture and Technology, Tokyo, Japan

3. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA

Abstract

Abstract Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIPsuc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.

Funder

Japan Society for the Promotion of Science

U.S. Department of Energy

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/80/1/162/36807342/bbb0162.pdf

Reference21 articles.

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2. The biochemistry of insect hemolymph;Wyatt;Annu. Rev. Entomol,1961