Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed-Reference-Cited by-同舟云学术

Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed

Published:2015-12-02 Issue:12 Volume:79 Page:2014-2017
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Miyanoshita Mitsuru¹,Nakashima Takashi¹²,Kakuta Yoshimitsu¹²,Kimura Makoto¹²

Affiliation:

1. Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Hakozaki 6-10-1, Fukuoka 812-8581, Japan

2. Laboratory of Biochemistry, Faculty of Agriculture, Department of Bioscience and Biotechnology, Graduate School, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan

Abstract

Abstract Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.

Funder

Ministry of Education, Culture, Sports, Science, and Technology of Japan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/79/12/2014/36820595/bbb2014.pdf

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