Preparation of proteoglycan from salmon nasal cartilage under nondenaturing conditions-Reference-Cited by-同舟云学术

Preparation of proteoglycan from salmon nasal cartilage under nondenaturing conditions

Published:2015-10-03 Issue:10 Volume:79 Page:1615-1618
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Tatara Yota¹²,Suto Shinichiro¹²,Sasaki Yoshitaka²,Endo Masahiko²

Affiliation:

1. Department of Glycotechnology, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, Hirosaki, Japan

2. Department of Glycobiomedicine, Hirosaki University Graduate School of Medicine, Hirosaki, Japan

Abstract

Abstract Salmon nasal cartilage was micronized in ethanol using a rotor–stator homogenizer for the high yield of proteoglycan extraction. This procedure also brought about depressing the degradation of proteoglycan and the contamination of collagens. Proteoglycan was extracted by 4 M magnesium chloride and isolated by anion-exchange chromatography. The gel filtration HPLC and the antibody reactivity showed that the core protein was intact.

Funder

Hirosaki University Grant for Exploratory Research by Young Scientists and by Sunstar Inc

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/79/10/1615/36837889/bbb1615.pdf

Reference14 articles.

1. Proteoglycan core protein families;Hassell;Ann. Rev. Biochem,1986

2. Extracellular matrix, supramolecular organisation and shape;Scott;J. Anat,1995

3. Proteoglycans: many forms and many functions;Hardingham;FASEB J,1992

4. Epiphycan from salmon nasal cartilage is a novel type of large leucine-rich proteoglycan;Tatara;Glycobiology,2013

5. Protein polysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures;Sajdera;J. Biol. Chem,1969

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Comprehensive analysis of chondroitin sulfate and aggrecan in the head cartilage of bony fishes: Identification of proteoglycans in the head cartilage of sturgeon;International Journal of Biological Macromolecules;2022-05

2. Bioactives From Seafood Processing By-Products;Encyclopedia of Food Chemistry;2019