Pinctada fucata mantle gene 4 (PFMG4) from pearl oyster mantle enhances osteoblast differentiation-Reference-Cited by-同舟云学术

Pinctada fucata mantle gene 4 (PFMG4) from pearl oyster mantle enhances osteoblast differentiation

Published:2015-04-03 Issue:4 Volume:79 Page:558-565
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Wang Xiaoyan¹²,Harimoto Kenichi¹³,Fuji Ryosuke¹³,Liu Jing¹,Li Liyuan¹,Wang Pan¹,Akaike Toshihiro³,Wang Zhao¹

Affiliation:

1. Protein Science Key Laboratory of the Ministry of Education, School of Medicine, Tsinghua University, Beijing, P.R. China

2. Department of Chemistry and Biology, School of Science, National University of Defense Technology, Changsha, P.R. China

3. Department of Biomolecular Engineering, School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan

Abstract

Abstract The organic matrix of nacre has been reported for its effect on osteogenesis. It was found that PFMG4 (Pinctada fucata mantle gene 4) with an N-terminal signal peptide could be secreted into nacre of Pinctada fucata (P. fucata). Here, we report that PFMG4 is highly expressed in mantle tissue and has high homology with C1q protein in different species. In MC3T3-E1 osteoblast cells, we found that highly expressed PFMG4 could suppress cell proliferation and type I collagen expression, but it could increase alkaline phosphatase activity and mineralized deposition. These results show that PFMG4 has potential ability in enhancing osteoblast differentiation, suggesting a new idea in developing medicine for the therapy of osteoporosis.

Funder

This work was financially supported by the National Basic Research Program (973 Project) of China [grant number 2007CB507406], the Tsinghua-Yue-Yuen Medical Sciences Fund [grant number THYY20070008], and National University of Defense Technology Yu-Yan Fund [grant number JC12-02-14].

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/79/4/558/36806333/bbb0558.pdf

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