TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation-Reference-Cited by-同舟云学术

TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation

Published:2017-11-02 Issue:11 Volume:81 Page:2164-2167
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Nakamura Minako¹,Kumrungsee Thanutchaporn¹,Sakuma Tetsushi²,Yamamoto Takashi²,Yanaka Noriyuki¹

Affiliation:

1. Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan

2. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan

Abstract

Abstract In this study, we investigated the physiological function of glycerophosphodiesterase 5 (GDE5) in the proliferation of NIH3T3 fibroblasts. We used transcription activator-like effector nuclease (TALEN) in NIH3T3 cells with an intron targeting-mediated GDE5 gene knockout. The heterozygously GDE5-targeted NIH3T3 fibroblasts were isolated and showed decreased cell proliferation and up-regulation of EGFR mRNA expression, indicating that GDE5 modulates fibroblastic cell proliferation.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/81/11/2164/36844198/bbb2164.pdf

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