Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription-Reference-Cited by-同舟云学术

Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription

Published:2017-12-02 Issue:12 Volume:81 Page:2307-2313
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Sato Kazuomi¹²,Takei Masahiro¹,Iyota Ray¹,Muraoka Yoshimasa¹,Nagashima Marika¹,Yoshimura Yoshitaka³²

Affiliation:

1. Department of Agri-Production Sciences, College of Agriculture, Tamagawa University, Machida, Tokyo, Japan

2. Graduate School of Agriculture, Tamagawa University, Tamagawa-gakuen, Machida, Tokyo, Japan

3. Department of Advanced Food Sciences, College of Agriculture, Machida, Tokyo, Japan

Abstract

Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit several divergent biological effects. In this study, we investigated the effect of indomethacin on melanin synthesis using B16F1 melanoma cells. Indomethacin inhibited α-melanocyte stimulating hormone (α-MSH)-enhanced melanin synthesis in a dose-dependent manner. Western blotting analysis revealed that indomethacin significantly suppressed tyrosinase and Mitf protein levels. In a luciferase reporter assay, we found that indomethacin reduced tyrosinase promoter activity. Moreover, real-time RT-PCR analysis showed that indomethacin lowered mRNA levels of melanogenic genes, including Mitf. Together, our findings indicate that indomethacin inhibits melanogenesis via the suppression of Mitf transcription.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/81/12/2307/36839507/bbb2307.pdf

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