Function of PoLAE2, a laeA homolog, in appressorium formation and cAMP signal transduction in Pyricularia oryzae

Author:

Prajanket Pradabrat1,Vu Kim-Chi Thi1,Arai Jun1,Sornkom Worawan1,Abe Ayumi2,Sone Teruo2

Affiliation:

1. Graduate School of Agriculture, Hokkaido University, Sapporo, Japan

2. Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan

Abstract

Abstract A novel homolog of laeA, a global regulatory gene in filamentous fungi, was identified from Pyricularia oryzae. A deletion mutant of the homolog (PoLAE2) exhibited lowered intracellular cAMP levels, and decreased appressorium formation on non-host surface; the decrease was recovered using exogenous cAMP and IBMX, indicating that PoLAE2 deletion affected the cAMP signaling pathway.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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