Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551-Reference-Cited by-同舟云学术

Expression of recombinant organophosphorus hydrolase in the original producer of the enzyme, Sphingobium fuliginis ATCC 27551

Published:2016-05-03 Issue:5 Volume:80 Page:1024-1026
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Nakayama Kosuke¹,Ohmori Takeshi²,Ishikawa Satoshi¹,Iwata Natsumi¹,Seto Yasuo²,Kawahara Kazuyoshi²

Affiliation:

1. Department of Biosciences, College of Science and Engineering, Kanto Gakuin University, Yokohama, Japan

2. Third Department of Forensic Science, National Research Institute of Police Science, Kashiwa, Japan

Abstract

Abstract The plasmid encoding His-tagged organophosphorus hydrolase (OPH) cloned from Sphingobium fuliginis was modified to be transferred back to this bacterium. The replication function of S. amiense plasmid was inserted at downstream of OPH gene, and S. fuliginis was transformed with this plasmid. The transformant produced larger amount of active OPH with His-tag than E. coli.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/80/5/1024/36828008/bbb1024.pdf

Reference17 articles.

1. Proposal of Sphingomonadaceae fam. nov., consisting of Sphingomonas Yabuuchi et al. 1990, Erythrobacter Shiba and Shimidu 1982, Erythromicrobium Yurkov et al. 1994, Porphyrobacter Fuerst et al. 1993, Zymomonas Kluyver and van Niel 1936, and Sandaracinobacter Yurkov et al. 1997, with the type genus Sphingomonas Yabuuchi et al. 1990.;Kosako;Microbiol. Immunol,1990

2. Chemical structure and function of glycosphingolipids of Sphingomonas spp. and their distribution among members of the & #x03B1;-4 subclass of Proteobacteria;Kawahara;J. Ind. Microbiol. Biotechnol,1999

3. Molecular characteristics of xenobiotic-degrading sphingomonads;Stolz;Appl. Micobiol. Biotechnol,2009

4. Metabolism of dibenzo-p-dioxin by Sphingomonas sp. strain RW1;Wittich;Appl. Environ. Microbiol,1992

5. Aerobic degradation of lindane (γ-hexachlorocyclohexane) in bacteria and its biochemical and molecular basis;Nagata;Appl. Microbiol. Biotechnol,2007

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Fusion proteins of organophosphorus hydrolase and pHluorin for a whole-cell biosensor for organophosphorus pesticide measurement;Analytical Sciences;2023-06-01

2. Applications of phytochemicals against nerve agents in counterterrorism;Phytochemistry, the Military and Health;2021

3. Microbial Detoxification of Residual Organophosphate Pesticides in Agricultural Practices;Microbial Biotechnology;2017