Affiliation:
1. Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University , Okayama, Japan
2. Faculty of Agriculture, Okayama University , Okayama, Japan
Abstract
Abstract
Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Mizutani Foundation for Glycoscience
Ministry of Education, Culture, Sports, Science, and Technology of Japan
Publisher
Oxford University Press (OUP)
Subject
Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology
Cited by
5 articles.
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