Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon-Reference-Cited by-同舟云学术

Establishment of plasmid vector and allelic exchange mutagenesis systems in a mycobacterial strain that is able to degrade polycyclic aromatic hydrocarbon

Published:2018-07-03 Issue:7 Volume:82 Page:1169-1171
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kishida Kouhei¹,Ogawa Natsumi¹,Ichihashi Eikichi¹,Kato Hiromi¹,Nagata Yuji¹,Ohtsubo Yoshiyuki¹,Tsuda Masataka¹

Affiliation:

1. Graduate School of Life Sciences, Tohoku University , Sendai, Japan

Abstract

Abstract Plasmid vector and allelic exchange mutagenesis systems were established for the genetic analysis of a phenanthrene-degrading mycobacterial strain, Mycobacterium sp. EPa45. Successful application of these systems revealed the necessity of the EPa45 phdI gene for the degradation of 1-hydroxy-2-naphthoate, which has been proposed to be an intermediate product in the degradation pathway of phenanthrene.

Funder

Japan Society for the Promotion of Science

a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/82/7/1169/36845174/bbb1169.pdf

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