Affiliation:
1. Faculty of Engineering, Department of Applied Chemistry and Biotechnology, Chiba University Nishi-Chiba Campus (Administration Bureau), Chiba-shi, Japan
Abstract
Abstract
The cellular activities of gymnosperms monoterpene synthases are largely compromised due to their requirement for manganese, which is deficient in microbial cells. Through site-saturation mutagenesis of the residue adjacent to metal-binding glutamate, we found that pinene synthase is highly mutable at this position yet drastically alter their metal binding preference, thereby quickly improving the cellular performance in heterologous hosts.
Funder
Ministry of Education, Culture, Sports, Science and Technology
Publisher
Oxford University Press (OUP)
Subject
Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology
Cited by
3 articles.
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