Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas

Author:

Fujii Sotaro12,Masanari-Fujii Misa1,Kobayashi Shinya1,Kato Chiaki3,Nishiyama Masayoshi4,Harada Yoshie5,Wakai Satoshi6,Sambongi Yoshihiro1

Affiliation:

1. Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan

2. Global Career Design Center, Hiroshima University, Higashi-Hiroshima, Japan

3. Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan

4. The HAKUBI Center for Advanced Research, Kyoto University, Kyoto, Japan

5. Institute for Integrated Cell–Material Sciences (WPI–iCeMS), Kyoto University, Kyoto, Japan

6. Graduate School of Science Technology and Innovation, Kobe University, Kobe, Japan

Abstract

Abstract Two cytochromes c5 (SBcytc and SVcytc) have been derived from Shewanella living in the deep-sea, which is a high pressure environment, so it could be that these proteins are more stable at high pressure than at atmospheric pressure, 0.1 MPa. This study, however, revealed that SBcytc and SVcytc were more stable at 0.1 MPa than at higher pressure. In addition, at 0.1–150 MPa, the stability of SBcytc and SVcytc was higher than that of homologues from atmospheric-pressure Shewanella, which was due to hydrogen bond formation with the heme in the former two proteins. This study further revealed that cytochrome c551 (PMcytc) of deep-sea Pseudomonas was more stable than a homologue of atmospheric-pressure Pseudomonas aeruginosa, and that specific hydrogen bond formation with the heme also occurred in the former. Although SBcytc and SVcytc, and PMcytc are phylogenetically very distant, these deep-sea cytochromes c are commonly stabilized through hydrogen bond formation.

Funder

Japanese Society for the Promotion of Science

Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3