Overexpressing of POU2F2 accelerates fracture healing via regulating HMGA1/Wnt/β-catenin signaling pathway-Reference-Cited by-同舟云学术

Overexpressing of POU2F2 accelerates fracture healing via regulating HMGA1/Wnt/β-catenin signaling pathway

Published:2020-03-03 Issue:3 Volume:84 Page:491-499
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Zhang Wei¹,Zhao Hanke²,Chen Kun³,Huang Ye³

Affiliation:

1. Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China

2. Department of Orthopedics, Changzhou Wujin People’s Hospital, Changzhou, China

3. Department of Orthopedics, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China

Abstract

ABSTRACT To elucidate the role of POU2F2 (POU class 2 homeobox 2) in fracture healing, 30 rats with femoral fracture were randomly grouped into three groups: FF group, LV-POU2F2 group and LV-scramble group. Rats were injected with PBS, lentivirus expressing POU2F2 or scramble lentivirus once a week for 4 weeks. Results showed that overexpressing of POU2F2 promoted fracture healing and callus growth. Besides, overexpressing of POU2F2 promoted protein and mRNA expression of Col10a1, Runx2, Osterix, and Osteocalcin. High Mobility Group AT-hook 1 (HMGA1) is a non-histone protein participating in chromatin remodeling of cells. Western blotting manifested HMGA1/Wnt/β-catenin pathway was activated in POU2F2 group. Moreover, in-vitro study of hMSCs cells supported the above data. In conclusion, POU2F2 promotes fracture healing via activating the HMGA1/Wnt/β-catenin signaling pathway.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/84/3/491/36825373/bbb0491.pdf

Reference40 articles.

1. Fracture healing: mechanisms and interventions;Einhorn;Nat Rev Rheumatol,2015

2. Fibrinolysis as a target to enhance fracture healing;O’Keefe;N Engl J Med,2015

3. Epidermal growth factor receptor (EGFR) signaling promotes proliferation and survival in osteoprogenitors by increasing early growth response 2 (EGR2) expression;Chandra;J Biol Chem,2013

4. Amphiregulin;Berasain;Semin Cell Dev Biol,2014

5. Griffin XL, Costa ML, Parsons N, et al. (2011) Electromagnetic field stimulation for treating delayed union or non-union of long bone fractures in adults, The Cochrane database of systematic reviews, CD008471.

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