Characterization of iron-sulfur clusters in flavin-containing opine dehydrogenase-Reference-Cited by-同舟云学术

Characterization of iron-sulfur clusters in flavin-containing opine dehydrogenase

Published:2016-12-01 Issue:12 Volume:80 Page:2371-2375
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Watanabe Seiya¹²,Tajima Kunihiko³,Matsui Kazuma³,Watanabe Yasuo¹

Affiliation:

1. Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Japan

2. Center for Marine Environmental Studies, Ehime University, Matsuyama, Japan

3. Department of Bio-molecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto, Japan

Abstract

Abstract Flavin-containing opine dehydrogenase from Bradyrhizobium japonicum forms a heterooligomeric α4β4γ4 enzyme complex. An electron paramagnetic resonance spectroscopy analysis using wild-type and site-directed mutants revealed that [4Fe-4S] and [2Fe-2S] clusters bind to two different types of [Fe-S] binding sites in the γ- and α-subunits, respectively. The latter was found to be important for structural folding and enzyme catalysis.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/80/12/2371/36828366/bbb2371.pdf

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