Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5-Reference-Cited by-同舟云学术

Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

Published:2018-12-02 Issue:12 Volume:82 Page:2094-2097
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Peng Zhao¹,Shibata Nao²,Tada Hideaki²,Kaneko Jun¹

Affiliation:

1. Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan

2. Exploratory Research Laboratories, Tsukuba Research Institute, ONO pharmaceutical Co., LTD, Tsukuba, Japan

Abstract

ABSTRACT CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

Funder

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/82/12/2094/36831237/bbb2094.pdf

Reference16 articles.

1. Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins: structures, pore-forming mechanism, and organization of the genes;Kaneko;Biosci Biotechnol Biochem,2004

2. Panton-Valentine leucocidin and gamma-hemolysin from Staphylococcus aureus ATCC 49775 are encoded by distinct genetic loci and have different biological activities;Prévost;Infect Immun,1995

3. Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family;Gravet;FEBS Lett,1998

4. Purification, cloning and characterization of variant LukE-LukD with strong leukocidal activity of staphylococcal bi-component leukotoxin family;Morinaga;Microbiol Immunol,2003

5. Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel;Olson;Nat Struct Biol,1999