Enhanced elevations of hypo-osmotic shock-induced cytosolic and nucleic calcium concentrations in tobacco cells by pretreatment with dimethyl sulfoxide

Author:

Nguyen Hieu T H1,Bouteau François23,Mazars Christian4,Kuse Masaki5,Kawano Tomonori123

Affiliation:

1. International Photosynthesis Industrialization Research Center, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Japan

2. Université Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France

3. LINV Kitakyushu Research Cente, University of Florence, Kitakyushu, Japan

4. Laboratoire de Recherches en Sciences Végétales, Université de Toulouse UPS, Castanet-Tolosan, France

5. Laboratory of Natural Products Chemistry, Graduate School of Agricultural Science, Kobe University, Kobe, Japan

Abstract

ABSTRACT Dimethyl sulfoxide (DMSO) is a dipolar aprotic solvent widely used in biological assays. Here, we observed that DMSO enhanced the hypo-osmotically induced increases in the concentration of Ca2+ in cytosolic and nucleic compartments in the transgenic cell-lines of tobacco (BY-2) expressing aequorin.

Funder

Ministry of Education, Culture, Sports, Science and Technology

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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