Ergothioneine production with Aspergillus oryzae-Reference-Cited by-同舟云学术

Ergothioneine production with Aspergillus oryzae

Published:2019-01-02 Issue:1 Volume:83 Page:181-184
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Takusagawa Shun¹,Satoh Yasuharu²,Ohtsu Iwao³,Dairi Tohru²

Affiliation:

1. Graduate School of Chemical Science and Engineering, Hokkaido University, Sapporo, Hokkaido, Japan

2. Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido, Japan

3. Innovation Medical Research Institute, University of Tsukuba, Tsukuba, Ibaraki, Japan

Abstract

ABSTRACT To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry

Funder

Grants-in-Aid for Scientific Research from JSPS

the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry from the Ministry of Agriculture, Forestry and Fisheries

Grants-in-Aid for Research on Innovative Areas from MEXT

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/83/1/181/36826333/bbb0181.pdf

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