Presence of osteoinductive factors in bovine colostrum

Author:

Mussano Federico1,Bartorelli Cusani Alberto2,Brossa Alessia3,Carossa Stefano1,Bussolati Gianni4,Bussolati Benedetta3

Affiliation:

1. Department of Surgical Sciences, Dental School, University of Turin, Torino, Italy

2. Clinical Immunology, University of Milan, Milano, Italy

3. Department of Molecular Biotechnology and Health Sciences, University of Turin, Torino, Italy

4. Department of Medical Sciences, University of Turin, Torino, Italy

Abstract

Abstract New approaches in the treatment of skeletal defects may benefit from the use of soluble biological factors. We previously standardized a derivative of bovine colostrum (SBCD), deprived of casein and fat and rich in cytokines. In the present study, we tested its possible use as an adjuvant in bone healing. SBCD contained factors involved in stromal cell stimulation and differentiation and induced cytokine production from stimulated mesenchymal stem cells (MSCs). In vitro, SBCD promoted proliferation, migration and, in association with osteogenic factors, osteogenic differentiation of osteoblastic and MSCs. In in vivo experiments of subcutaneous Matrigel injection in mice, SBCD plus hydroxyapatite, but not hydroxyapatite nor SBCD alone, induced recruitment of macrophages and stromal cells. After 60 days, plugs containing SBCD and hydroxyapatite were densely calcified and diffusely positive for osteocalcin, supporting the occurrence of an early osteogenic process. These results indicate that SBCD is a rich source of factors with osteoinductive properties.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Reference35 articles.

1. Bone substitutes: an update;Giannoudis;Injury,2005

2. Bone grafts;Hubble;Surg. Technol. Int,2002

3. Harvesting autogenous iliac bone grafts;Kurz;Spine,1989

4. Chronic donor site pain complicating bone graft harvesting from the posterior iliac crest for spinal fusion;Femyhough;Spine,1992

5. Bone grafts;Solomon;J. Bone Joint. Surg. Br,1991

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