Biodegradation characterization and immobilized strains’ potential for quinoline degradation by Brevundimonas sp. K4 isolated from activated sludge of coking wastewater

Abstract

Abstract A novel quinoline-degrading strain, named K4, was isolated from activated sludge of a coking wastewater treatment plant and identified as Brevundimonas sp. on the basis of its 16s rDNA gene sequence analysis. Its optimum temperature and pH for quinoline degradation were 30 °C and pH 9.0, respectively, and during the biodegradation process, at 100 mg/L initial quinoline concentration, an inoculation amount of 8% (OD600 of 0.23) was the optimal strain concentration. In addition, the kinetics of free K4 strains for quinoline degradation showed that it followed a zero-order equation. Furthermore, compared with free K4 strains, immobilized K4 strains’ potential for quinoline degradation was investigated by adding both of them into SBR reactors for actual coking wastewater treatment on operation over 15 days. The results showed that bioaugmentation by both free and immobilized K4 strains enhanced quinoline removal efficiency, and especially, the latter could reach its stable removal after a shorter accommodation period, with 94.8% of mean quinoline removal efficiency.