Imperatorin alleviates ROS-mediated airway remodeling by targeting the Nrf2/HO-1 signaling pathway

Author:

Xian Zhemin1,Choi Yun Ho2,Zheng Mingyu3,Jiang Jingzhi4,Zhao Yuzhe1,Wang Chongyang4,Li Junfeng4,Li Yan1,Li Liangchang4,Piao Hongmei1,Yan Guanghai4

Affiliation:

1. Department of Respiratory Medicine, Affiliated Hospital of Yanbian University, Yanji, P.R. China

2. Department of Anatomy, Medical School, Institute for Medical Sciences, Chonbuk National University, Jeonju, Republic of Korea

3. College of Pharmacy, Yanbian University, Yanji, P.R. China

4. Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, P.R. China

Abstract

Abstract In this study, we investigated the role and mechanism of imperatorin (IMP) in chronic inflammation and airway remodeling. The levels of TNF-α, IL-1β, IL-6, IL-8, VEGF, α-SMA, and ROS were detected by ELISA, immunohistochemistry (IHC), immunofluorescence, and Western blot. In addition, we evaluated the effect of IMP on MAPK, PI3K/Akt, NF-κB, and Nrf2/HO-1 signaling pathways. IMP treatment obviously attenuated the production of inflammatory cytokines and inflammatory cells in bronchoalveolar lavage fluid of OVA-induced airway remodeling model. Meanwhile, it significantly inhibited inflammatory cell infiltration, goblet cell hyperplasia, collagen deposition, VEGF production, α-SMA, and ROS expression. Our study has shown that IMP could regulate the signaling pathways including MAPK, PI3K/Akt, NF-κB, and Nrf2/HO-1 to release the inflammatory responses. IMP might attenuate airway remodeling by the down-regulation of Nrf2/HO-1/ROS/PI3K/Akt, Nrf2/HO-1/ROS/MAPK, and Nrf2/HO-1/ROS/NF-κB signaling pathways.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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