Enhanced sugar chain detection by oriented immobilization of Fc-fused lectins-Reference-Cited by-同舟云学术

Enhanced sugar chain detection by oriented immobilization of Fc-fused lectins

Published:2020-09-01 Issue:9 Volume:84 Page:1775-1779
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Iijima Masumi¹²,Yamada Yuki¹,Nakayama Tsutomu²,Kuroda Shun'ichi¹

Affiliation:

1. The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan

2. Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya, Tokyo, Japan

Abstract

Abstract We report a novel scaffold for clustering and oriented immobilization of human IgG1 Fc-fused lectins on biosensors without chemical modifications. This approach uses a bio-nanocapsule (BNC) displaying a tandem form of IgG Fc-binding Z domains derived from Staphylococcus aureus protein A (ZZ-BNC). Incorporating ZZ-BNC effectively increased both the sensitivity and sugar chain-binding capacity compared with the condition without ZZ-BNC.

Funder

KAKENHI Grant-in-Aid for Scientific Research

Japan Agency for Medical Research and Development

“Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials” from the Ministry of Education, Culture, Sports, Science and Technology of Japan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/84/9/1775/36826811/bbb1775.pdf

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