Aggregability of β(1→4)-linked glucosaminoglucan originating from a sulfur-oxidizing bacterium Thiothrix nivea

Author:

Takeda Minoru1,Kondo Keiko2,Tominaga Ryo1,Mori Honomi1,Kato Mana1,Usami Ryoji1,Murakami Tomoi1,Ueda Kazuyoshi1,Suzuki Ichiro1,Katahira Masato23

Affiliation:

1. Graduate School of Engineering, Yokohama National University, Yokohama, Japan

2. Institute of Advanced Energy, Kyoto University, Kyoto, Japan

3. Graduate School of Energy Science, Kyoto University, Kyoto, Japan

Abstract

Abstract β-1,4-glucosaminoglucan (GG) was prepared from the sheath of a sulfur-oxidizing bacterium Thiothrix nivea. Recently, GG was found to be adsorbed by cellulose (paper) and is therefore potentially applicable as an aminating agent for cellulose. We attempted to increase the yield of GG using a fed-batch cultivation method. Furthermore, the behavior of GG molecules in water was theoretically and experimentally investigated. NMR analysis in combination with molecular dynamics calculation suggested that GG molecules tend to form soluble aggregates in water. It was experimentally revealed that the self-aggregation is enhanced by the addition of NaCl and reduced temperature. Adsorption of GG onto cellulose via hydrogen bonding was confirmed by molecular dynamics simulation. Adsorption was also promoted in the presence of NaCl but was inhibited by a reduction in temperature. Only 11% of the amino groups in the GG-treated paper was reactive, suggesting that GG molecules adsorbed by the paper were forming aggregates.

Funder

Kyoto University

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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