5-Keto-D-fructose production from sugar alcohol by isolated wild strain Gluconobacter frateurii CHM 43

Author:

Adachi Osao1,Nguyen Thuy M1,Hours Roque A2,Kataoka Naoya1,Matsushita Kazunobu1,Akakabe Yoshihiko1,Yakushi Toshiharu1

Affiliation:

1. Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan

2. CINDEFI, School of Science, La Plata National University, La Plata, Argentina

Abstract

Abstract Gluconobacter frateurii CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydrogenase) and flavoprotein D-fructose dehydrogenase in the membranes. When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells. The method proposed here should give a smart strategy for 5-keto-D-fructose production.

Funder

Yamaguchi University Fund

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Reference16 articles.

1. Formation of 5-dehydrofructose by members of Acetobacter;Terada;Bull Agric Chem Soc Jpn,1960

2. Formation of 5-dehydrofructose by members of Acetobacter species. Part I. Formation of a new strong reducing substance from fructose and its discrimination from glucosone;Terada;Bull Agric Chem Soc Jpn,1961

3. Formation of 5-dehydrofructose by members of Acetobacter species. Part II. Studies on the fermentation, purification and properties of crystal;Terada;Bull Agric Chem Soc Jpn,1961

4. Formation of 5-dehydrofructose by members of Acetobacter species. Part III. Characterization of the unknown substance;Terada;Bull Agric Chem Soc Jpn,1961

5. 5-Keto-D-fructose: formation and utilization in the course of D-fructose assimilation by Gluconobacter cerinus;Mowshowitz;J Biol Chem,1974

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