Affiliation:
1. Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
Abstract
Abstract
α-1,4-Glucan lyases [glycoside hydrolase family (GH) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (AF), while GH31 α-glucosidases normally catalyze a hydrolytic reaction. We determined that a small amount of AF was produced by GH31 Aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate.
Publisher
Oxford University Press (OUP)
Subject
Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology
Reference26 articles.
1. α-1,4-Glucan lyase, a new starch processing enzyme for production of 1,5-anhydro-d-fructose;Yu;Zuckerindustrie,2004
2. α-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds;Yu;Biochim. Biophys. Acta,1993
3. α-1,4-Glucan lyase, a new class of starch/glycogen-degrading enzyme. II. Subcellular localization and partial amino acid sequence;Yu;Planta,1993
4. Characterization of exo-(1,4)-α glucan lyase from red alga Gracilaria chorda. Activation, inactivation and the kinetic properties of the enzyme;Yoshinaga;Biochim. Biophys. Acta,1999
5. N-Linked oligosaccharide processing enzyme glucosidase II produces 1,5-anhydrofructose as a side product;Hirano;Glycobiology,2000
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献