A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney-Reference-Cited by-同舟云学术

A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney

Published:2003-06 Issue:4 Volume:31 Page:462-464
ISSN:0192-6233
Container-title:Toxicologic Pathology
language:en
Short-container-title:Toxicol Pathol

Author:

De Rijk Eveline P.C.T.¹,Ravesloot Wilma T.M.²,Wijnands Yvonne²,Van Esch Eric²

Affiliation:

1. Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands,

2. Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands

Abstract

Hyaline droplet formation in the proximal tubular cells of the kidney commonly occurs under different pathological conditions in experimental animals. In rats, intracytoplasmic hyaline droplet formation is predominantly associated with accumulation of the male rat-specific α2u-globulin, whereas under other (pathological) conditions (eg, histiocytic sarcoma and chronic progressive nephropathy [CPN]) other proteins are involved. Staining methods to visualize hyaline droplets either need plastic embedded material or time-consuming (immuno)histochemical methods. A fast Chromotrope-Aniline-Blue-staining on formalin-fixed paraffin-embedded kidneys taking only 30 minutes is described. Using this method, hyaline droplets consisting of different types of proteins are easily recognized by their bright-red color.

Publisher

SAGE Publications

Subject

Cell Biology,Toxicology,Molecular Biology,Pathology and Forensic Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1080/01926230390213775

Reference12 articles.

1. A Review of Unique Male Rat Hydrocarbon Nephropathy

2. Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple Stains

3. Localization of alpha 2u-globulin within protein droplets of male rat kidney: immunohistochemistry using perfusion-fixed, GMA-embedded tissue sections.

4. Retrospective evaluation of alpha 2u-globulin accumulation in male rat kidneys following high doses of diisononyl phthalate

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