1. Discrepancies Between Radioimmunoassays and Bioassay for Rat FSH: Evidence that Androgen Treatment and Withdrawal Can Alter Biossay—Immunoassay Ratios1
2. Gonadal Function and the Biological and Physicochemical Properties of FollicleZ— Stimulating Hormone
3. The NIAMD radioimmunoassays for rat LH and rat TSH, and the DY-2 assay for rat FSH (1), were used in these studies. These assays all react, to some extent, with other pituitary glycoprotein hormones. We examined the extent to which such cross-reactions might have influenced our findings. The asymmetry of the curves shown in Figs. 1–6 could have been due to overestimation of the descending limbs of the FSH and LH curves, and of the ascending limbs of the TSH curves. However, in the peak regions of these elution profiles, the possible errors due to cross-reaction were small. FSH concentrations at and near the peaks shown in Fig. 6 were recalculated on the basis of known LH contamination (Fig. 5) and a maximum estimate of LH cross-reaction (1). Corrections based on this outside estimate of error did not alter the elution positions of any of these profiles significantly