Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets-Reference-Cited by-同舟云学术

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

Published:2020-01-01 Issue:1 Volume:9 Page:1175-1179
ISSN:2222-1751
Container-title:Emerging Microbes & Infections
language:en
Short-container-title:Emerging Microbes & Infections

Author:

Liu Xinjin¹,Feng Jiangpeng¹,Zhang Qiuhan¹,Guo Dong¹,Zhang Lu¹,Suo Tao²,Hu Wenjia³,Guo Ming¹,Wang Xin¹,Huang Zhixiang¹,Xiong Yong³^ORCID,Chen Guozhong²,Chen Yu¹^ORCID,Lan Ke¹⁴

Affiliation:

1. State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China

2. State Key Laboratory of Virology, Renmin Hospital, Wuhan University, Wuhan, People’s Republic of China

3. Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People’s Republic of China

4. Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan, People’s Republic of China

Funder

COVID-19 Research of Wuhan University

Publisher

Informa UK Limited

Subject

Virology,Infectious Diseases,Drug Discovery,General Medicine,Immunology,Microbiology,Parasitology,Epidemiology

Link

https://www.tandfonline.com/doi/pdf/10.1080/22221751.2020.1772679

Reference11 articles.

1. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak

2. A distinct name is needed for the new coronavirus

3. Suo T, Liu X, Feng J, et al. ddPCR: a more sensitive and accurate tool for SARS-CoV-2 detection in low viral load specimens. medRxiv [Internet]. 2020;2020.02.29.20029439. Available from: https://medrxiv.org/content/early/2020/03/06/2020.02.29.20029439.abstract

4. Vogels CBF, Brito AF, Wyllie AL, et al. Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays. medRxiv [Internet]. 2020;2020.03.30.20048108. Available from: https://medrxiv.org/content/early/2020/04/01/2020.03.30.20048108.abstract

5. National Institute for Viral Disease Control and Prevention of PRC. Specific primers and probes for detection 2019 novel coronavirus [Internet]. 2020 [cited 2020 Apr 10]. Available from: https://www.chinaivdc.cn/kyjz/202001/t20200121_211337.html

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