Evaluating the feasibility of Cas9 overexpression in 3T3-L1 cells for generation of genetic knock-out adipocyte cell lines
Author:
Affiliation:
1. Department of Molecular Biochemistry, Rudolf Schönheimer Institute of Biochemistry, Medical Faculty, Leipzig University, Leipzig, Germany
2. Department of Evolutionary Genetics, Max-Planck-Institute for Evolutionary Anthropology, Leipzig, Germany
Funder
Deutsche Forschungsgemeinschaft
European Social Fund
Publisher
Informa UK Limited
Subject
Cell Biology,Histology
Link
https://www.tandfonline.com/doi/pdf/10.1080/21623945.2021.1990480
Reference55 articles.
1. Alternatives to animal testing: A review
2. Multiplex Genome Engineering Using CRISPR/Cas Systems
3. Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review
4. An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversion
5. Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches
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2. Protocol for changing gene expression in 3T3-L1 (pre)adipocytes using siRNA-mediated knockdown;STAR Protocols;2024-06
3. The genetically modified human foreskin fibroblast cell line (YhFF#8) stably expressing Cas9 gene: A lab resource report;International Journal of Reproductive BioMedicine (IJRM);2024-02-18
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