A rapid and simple procedure for the whole-mount bone staining of small fish

Author:

Yeesan Narawadee1,Sudtongkong Chanyut1,Kong-Oh Supparat1,Angsujinda Kitipong2,Kettratad Jes3,Poolprasert Pisit4,Iida Atsuo5,Boonyoung Piyakorn6,Nganvongpanit Korakot7,Kenthao Anan8,Kaneko Gen9,Senarat Sinlapachai110ORCID

Affiliation:

1. Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, Thailand

2. Aquatic Resources Research Institute, Chulalongkorn University, Bangkok, Thailand

3. Department of Marine Sciences, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

4. Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand

5. Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan

6. Division of Health and Applied Science, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand

7. Research Center for Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand

8. Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand

9. College of Natural and Applied and Science, University of Houston – Victoria, Victoria, TX, USA

10. Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand

Publisher

Informa UK Limited

Reference20 articles.

1. Ueber herstellung und conservirung durchsichtiger embryonen zum stadium der skeletbildung;Schultze O.;Anat Anz,1897

2. Kardong KV. Vertebrates: comparative anatomy, function, and evolution. 3rd ed. New York (NY): McGraw-Hill Higher Education; 2002.

3. An Enzyme Method of Clearing and Staining Small Vertebrates

4. Visualization of the fetal skeletal system by double staining with Alizarin Red and Alcian Blue;Erdogan D;Gazi Med J,1995

5. A rapid method for clearing and staining specimens for the demonstration of bone;Green MC;Ohio J Sci,1952

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