Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis-Reference-Cited by-同舟云学术

Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis

Published:2019-10-01 Issue:8 Volume:11 Page:1452-1463
ISSN:1942-0862
Container-title:mAbs
language:en
Short-container-title:mAbs

Author:

Harris Claire¹^ORCID,Xu Weichen²^ORCID,Grassi Luigi¹^ORCID,Wang Chunlei²^ORCID,Markle Abigail²,Hardman Colin³^ORCID,Stevens Richard⁴,Miro-Quesada Guillermo⁵,Hatton Diane¹,Wang Jihong²^ORCID

Affiliation:

1. Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK

2. Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA

3. Data Science & Artificial Intelligence, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK

4. Antibody Discovery and Protein Engineering, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK

5. Data & Quantitative Sciences, Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA

Publisher

Informa UK Limited

Subject

Immunology,Immunology and Allergy

Link

https://www.tandfonline.com/doi/pdf/10.1080/19420862.2019.1667740

Reference54 articles.

1. Maintaining consistent quality and clinical performance of biopharmaceuticals

2. Determination of critical quality attributes for monoclonal antibodies using quality by design principles

3. Amino acid misincorporation in recombinant proteins

4. Identification of multiple serine to asparagine sequence variation sites in an intended copy product of LUCENTIS® by mass spectrometry

5. Identification and characterization of a -1 reading frameshift in the heavy chain constant region of an IgG1 recombinant monoclonal antibody produced in CHO cells

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