When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions

Author:

Bradbury Andrew R. M.1,Trinklein Nathan D.2,Thie Holger3ORCID,Wilkinson Ian C.4,Tandon Atul K.5,Anderson Stephen4,Bladen Catherine L.4,Jones Brittany5,Aldred Shelley Force2,Bestagno Marco6,Burrone Oscar6,Maynard Jennifer7,Ferrara Fortunato1,Trimmer James S.8ORCID,Görnemann Janina9,Glanville Jacob10,Wolf Philipp11,Frenzel Andre1213,Wong Julin14,Koh Xin Yu14,Eng Hui-Yan14,Lane David14,Lefranc Marie-Paule15,Clark Mike16,Dübel Stefan13ORCID

Affiliation:

1. Specifica Inc., 1512 Pacheco St, Santa Fe, NM, USA

2. SwitchGear Genomics, 1914 Palomar Oaks Way, Carlsbad, CA USA

3. Miltenyi Biotec GmbH, Friedrich-Ebert-Str. 68, Bergisch Gladbach, Germany

4. Absolute Antibody, Wilton Centre, Redcar, Cleveland TS10 4RF, United Kingdom

5. NeoBiotechnologies, 2 Union Square, Union City, CA, USA

6. International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, Trieste, Italy

7. The University of Texas at Austin, Cockrell School of Engineering, McKetta Department of Chemical Engineering, 200 E Dean Keeton St. Stop C0400, Austin, Texas, USA

8. Department of Physiology and Membrane Biology, University of California, Davis, One Shields Avenue, Davis, CA, USA

9. Institute for Molecular Genetics, University of Heidelberg, Im Neuenheimer Field 260, Heidelberg, Germany

10. Stanford University, School of Medicine, Stanford, California, USA

11. Department of Urology, Medical Center, University of Freiburg, Breisacher Str. 66, Freiburg, Germany

12. Yumab GmbH, Inhoffenstr. 7, Braunschweig, Germany

13. Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Spielmannstr. 7, Braunschweig, Germany

14. A*Star p53 laboratory, 06-06 Immunos, Singapore, Singapore

15. IMGT®, the international ImMunoGeneTics information system®, Laboratoire d'ImmunoGénétique Moléculaire LIGM, Institut de Génétique Humaine IGH, UPR CNRS 1142, Montpellier University, Montpellier cedex 5, France

16. Clark Antibodies Ltd, 10 Wellington Street, Cambridge, CB1 1HW, United Kingdom

Publisher

Informa UK Limited

Subject

Immunology,Immunology and Allergy

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