Jesu li testovi praćenja oksidacijskog stresa dovoljni za genetički monitoring hrasta lužnjaka u malom razmjeru?

Abstract

Although being present much widely in terms of its geographical distribution, pedunculate oak (Quercus robur L.) represents one of the crucial species in forest ecosystems in South Eastern Europe. We wanted to contribute to existing efforts in designing genetic monitoring methodology for this noble broadleaved species, through the screening of genotypes with different health status and belonging to two phenological varieties [early (var. praecox) vs late (var. tardissima)] using seven oxidative stress parameters (total protein content, total phenolic compounds content, total flavonoids content, DPPH, ABTS, NO radical scavenger activities and Ferric reducing antioxidant power essay - FRAP). Results of this study provided significant biological message, contributing to better understanding of existing biodiversity of pedunculate oak in Serbia. Four out of seven oxidative stress screening tests (total phenolic compounds content, total flavonoids content, RSC NO and FRAP) clearly discriminated late vs early individuals sampled from selected pedunculate oak gene pool. Although presented results did not show significancy in using mentioned parameters to distinct damaged from vital individuals, nevertheless, knowledge gained through discussion and comparison with previous studies contributed to precise determination of biochemical tests which might be used in future efforts of genetic monitoring of this species.