Metabolic and radiofrequency features of reactive oxygen species and nitric oxide effects on biological tissue ex vivo-Reference-Cited by-同舟云学术

Metabolic and radiofrequency features of reactive oxygen species and nitric oxide effects on biological tissue ex vivo

Published:2021-09-26 Issue:3 Volume:6 Page:8-14
ISSN:2542-0941
Container-title:Fundamental and Clinical Medicine
language:
Short-container-title:Fundamentalʹnaâ i kliničeskaâ medicina

Author:

Martusevich A. K.¹^ORCID,Galka A. G.²^ORCID,Golygina E. S.¹^ORCID,Fedotova A. S.¹^ORCID

Affiliation:

1. Privolzhsky Research Medical University

2. Privolzhsky Research Medical University; Institute of Applied Physics, Russian Academy of Sciences

Abstract

Aim. To assess the effects of reactive oxygen species and nitric oxide on the scar tissue ex vivo.Materials and Methods. The study was performed using fragments of scar tissue (n = 10) removed intraoperatively in patients with Dupuytren's contracture. Each fragment was divided into 3 equal segments: 1) control; 2) treated with singlet oxygen for 5 minutes; 3) treated with nitric oxide (20 ppm) for 5 minutes. Upon the indicated treatment, we evaluated the dielectric properties of the tissue employing nearfield resonant microwave sensing using a customised software package (Institute of Applied Physics, Russian Academy of Sciences). Then, each segment was homogenised and the parameters of oxidative metabolism (intensity of free radical oxidation and total antioxidant activity) were measured in homogenates by Fe-induced biochemiluminescence.Results. Treatment of scar tissue fragments by singlet oxygen and nitric oxide altered the dielectric properties of the tissue and the intensity of free radical oxidation. Singlet oxygen action moderately increased the dielectric permittivity of the tissue and rendered a balanced stimulating effect on proand antioxidant systems. Nitric oxide significantly augmented dielectric permittivity and conductivity and raised the antioxidant potential of the tissue.

Publisher

Kemerovo State Medical University

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