Effect of different dry period duration on milk components and serum metabolites, and their associations with the first conception rate in multiparous Holstein dairy cows

Abstract

The aims of the present study were to evaluate the effects of different dry period (DP) lengths on milk fat to protein ratio (FPR) and metabolic status – blood leptin, adiponectin and non-esterified fatty acids (NEFA) concentrations in dairy cows, and their associations with the result of the first timed artificial insemination (TAI). Cows were blocked either to short DP (SDP; 30±2 days; n=72) or conventional DP (CDP; 60±2 days; n=76). Milk FPR was calculated at 30 and 60 days in milk (DIM). Body condition score (BCS) was recorded at –60, –30, calving, and 60 DIM. Blood samples were obtained at –60, –30, –7, calving, +7, +30, and +60 DIM for serum metabolites measurement. TAI was implemented between 65–75 DIM for all cows. Milk FPR and its changes were statistically analysed using an independent sample t test. To assess the impact of time, the pattern of BCS, and serum metabolites on the result of the first AI, repeated measure ANOVA was used. Only FPR-30 DIM revealed significant difference between pregnant and non-pregnant cows in SDP group (P<0.01). Reduced BCS loss was observed in the SDP group and followed by slightly higher probability of pregnancy at first AI (P=0.19). Leptin was not altered by shortening the DP (P≥0.1). Significant differences were observed in blood adiponectin prepartum (P<0.001) and at +7 DIM (P<0.01), as well as in NEFA at +7 and +30 DIM between the two groups (P<0.05). Pregnant cows following the first AI had significantly high postpartum leptin concentrations (P<0.05), high prepartum adiponectin (P≤0.001), and lower NEFA at +7 DIM (P<0.01) in the SDP group. In conclusion, shortening the dry period caused reduced BCS loss postpartum and variations in serum metabolits that favoured the possibility of pregnancy at first AI.