Modified, innovative method for detection of adulteration of meat pro­ducts with meat of other species

Author:

Tolba A. O.1,Abd-El-Aziz D. M.2,El-Sharkawy E. E.3,AbdelHafe E. A.4,Youssef H.2

Affiliation:

1. Food Hygiene, Assiut University Hospitals, Assiut University, Eg

2. Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Egypt

3. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medi-cine, Assiut University, Egypt

4. Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Egypt

Abstract

The aim of this study was to detect illegal adulteration of beef meat products with meat from other species. Samples (n=120) of industrial and handmade beef products were randomly collected from retail outlets in Assiut city, Egypt: raw beef burger, oriental beef sausage, beef kofta, and beef luncheon (30 samples each). Samples were analysed using agar gel immunodiffusion (AGID) and modified AGID (MAGID) assays. Results showed that 17.5% of examined products were adulterated with chicken meat. MAGID detected that 14.1% of samples were adulterated with donkey meat, whereas all AGID results were negative. Human tissue was detected in 8.3% (AGID) and 10% (MAGID) of examined samples. Histological examination was then used to detect foreign tissue, and all categories of products were found to be adulterated, and some of them - contaminated with human blood cells. Polymerase chain reaction analysis confirmed that MAGID was more accurate and sensitive than AGID, especially for false negative AGID results. Consumers are advised not to consume too much of the studied meat products to avoid exposure to adulterated or contaminated products that might constitute a health hazard.

Publisher

Trakia University

Subject

General Veterinary

Reference51 articles.

1. Abd El-Aziz, D. M., 2009. Identification of some meat species and non- meat protein in some meat products. PhD Thesis, Department of Food Hygiene and Control. Faculty of Veterinary Medicine, Assuit University, Egypt

2. Ahmed, H., M. El-Nasser, D. Mohammed, M. Mohamed, J. Köfer, & H. Schobes-berger, 2011. Identification of meat species in some raw meat products in Assiut city, Egypt. Animal hygiene and sustainable livestock production. In: Proceedings of the XVth International Congress of the International Society for Animal Hygiene, Vienna, Austria, 2, pp. 973975.

3. Ali, M. E., M. A. Razzak & S. B. A. Hamid, 2014. Multiplex PCR in species authentication: Probability and prospects  a review. Food Analytical Methods, 7, 19331949.

4. Al-Nassir, H. S., R. A. Kassim &B. H. Muhammed, 2014. Incidence of illegal substitution of beef with inferior-quality animal species meats sold in Kerbala, Basrah. Journal of Veterinary Research, 1, 108115.

5. Ayaz, Y., N. D. Ayaz & I. Erol, 2006. Detection of species in meat and meat products using Enzyme-Linked Immunosorbent Assay. Journal of Muscle Foods, 17, 214220.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3