In vitro effect of tetracycline antibiotics on Trueperella pyogenes isolated from cows with metritis

Abstract

Trueperella pyogenes is associated with endometritis and metritis in cows. Traditionally these diseases are treated with antibiotics while new approaches include application of the mucolytic N-acetylcysteine. Therefore the study aimed to evaluate the sensitivity of clinical Trueperella pyogenes isolates (n=2) to oxytetracycline, doxycycline, N-acetylcysteine and their combinations. The potential for biofilm formation with/without tested drugs was investigated by the method of crystal violet staining. Minimum inhibitory concentrations (MIC) of oxytetracycline for T. pyogenes 1 and 2 were 16 and 64 µg/mL, respectively. MIC of doxycycline for both isolates was 32 µg/mL and for N-acetylcysteine - 8 mg/mL. Both Trueperella pyogenes isolates did not form biofilm. The growth of T. pyogenes 1 cultured in the presence of either oxytetracycline or doxycycline (0.0078–128 µg/mL) under conditions for biofilm formation was significantly inhibited at concentrations ≥ 1 μg/mL and 8 μg/mL, respectively. The growth of T. pyogenes 2 was not affected by the antibiotics. N-acetylcysteine at ≥ 4 mg/mL resulted in significant inhibition of the growth of both isolates and its combinations with the antibiotics did not inhibit their growth. The effect of N-acetylcysteine should be validated in clinical settings but its combinations with tetracyclines were not able to improve the sensitivity of T. pyogenes, isolated from cows with clinical metritis.