Author:
Innocenti Nicolas,Golumbeanu Monica,Fouquier d'Hérouël Aymeric,Lacoux Caroline,Bonnin Rémy A.,Kennedy Sean P.,Wessner Françoise,Serror Pascale,Bouloc Philippe,Repoila Francis,Aurell Erik
Abstract
Enterococcus faecalis is the third cause of nosocomial infections. To obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified RNA-seq approach enabling to discriminate primary from processed 5′ RNA ends. We also validated our approach by confirming known features in Escherichia coli. We mapped 559 transcription start sites (TSSs) and 352 processing sites (PSSs) in E. faecalis. A blind motif search retrieved canonical features of SigA- and SigN-dependent promoters preceding transcription start sites mapped. We discovered 85 novel putative regulatory RNAs, small- and antisense RNAs, and 72 transcriptional antisense organizations. Presented data constitute a significant insight into bacterial RNA landscapes and a step toward the inference of regulatory processes at transcriptional and post-transcriptional levels in a comprehensive manner.
Funder
Swedish Science
Academy of Finland
German Academic Exchange Service
Publisher
Cold Spring Harbor Laboratory
Cited by
42 articles.
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