Author:
Burke Jordan E.,Sashital Dipali G.,Zuo Xiaobing,Wang Yun-Xing,Butcher Samuel E.
Abstract
The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended “Y” shape. The U6 internal stem–loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex—including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites—localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis.
Publisher
Cold Spring Harbor Laboratory
Cited by
80 articles.
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