Author:
BARANOV PAVEL V.,GESTELAND RAYMOND F.,ATKINS JOHN F.
Abstract
The expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed frameshifting. The present work extends this to other cases. When the A-site is unoccupied, the P-site tRNA can be repositioned forward with respect to mRNA (although repositioning in the minus direction is also possible). A kinetic model is presented for the influence of both, the cognate tRNAs competing for overlapping codons in A-site, and the stabilities of P-site tRNA:mRNA complexes in the initial and new frames. When the A-site is occupied, the P-site tRNA can be repositioned backward. Whether frameshifting will happen depends on the ability of the A-site tRNA to subsequently be repositioned to maintain physical proximity of the tRNAs. This model offers an alternative explanation to previously published mechanisms of programmed frameshifting, such as out-of-frame tRNA binding, and a different perspective on simultaneous tandem tRNA slippage.
Publisher
Cold Spring Harbor Laboratory
Reference58 articles.
1. Normal tRNAs promote ribosomal frameshifting
2. Atkins, J.F., Herr, A.J., Massire, C., O’Connor, M., Ivanov, I., and Gesteland, R.F. 2000. Poking a hole in the sanctity of the triplet code: Inferences for framing. In The ribosome: Structure, function, antibiotics and cellular interactions (eds. R.A. Garrett et al.), pp. 369–383. ASM Press, Washington, DC.
3. Recoding: translational bifurcations in gene expression
4. Release factor 2 frameshifting sites in different bacteria
5. RECODE 2003
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