Author:
DI SEGNI GIANFRANCO,BORGHESE LODOVICA,SEBASTIANI SILVIA,TOCCHINI-VALENTINI GLAUCO P.
Abstract
Archaeal pre-tRNAs are characterized by the presence of the bulge–helix–bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNATyr. This pre-tRNAArchEuka is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.
Publisher
Cold Spring Harbor Laboratory
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