Comparative study of the effects of heptameric slippery site composition on −1 frameshifting among different eukaryotic systems

Author:

PLANT EWAN P.,DINMAN JONATHAN D.

Abstract

Studies of programmed −1 ribosomal frameshifting (−1 PRF) have been approached over the past two decades by many different laboratories using a diverse array of virus-derived frameshift signals in translational assay systems derived from a variety of sources. Though it is generally acknowledged that both absolute and relative −1 PRF efficiency can vary in an assay system-dependent manner, no methodical study of this phenomenon has been undertaken. To address this issue, a series of slippery site mutants of the SARS-associated coronavirus frameshift signal were systematically assayed in four different eukaryotic translational systems. HIV-1 promoted frameshifting was also compared between Escherichia coli and a human T-cell line expression systems. The results of these analyses highlight different aspects of each system, suggesting in general that (1) differences can be due to the assay systems themselves; (2) phylogenetic differences in ribosome structure can affect frameshifting efficiency; and (3) care must be taken to employ the closest phylogenetic match between a specific −1 PRF signal and the choice of translational assay system.

Publisher

Cold Spring Harbor Laboratory

Subject

Molecular Biology

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