The transient expression of CHIKV VLP in large stirred tank bioreactors
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s10616-019-00346-x.pdf
Reference59 articles.
1. Akahata W et al (2010) A VLP vaccine for epidemic chikungunya virus protects non-human primates against infection. Nat Med 16:334–338. https://doi.org/10.1038/nm.2105
2. Ansorge S, Lanthier S, Transfiguracion J, Durocher Y, Henry O, Kamen A (2009) Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures. J Gene Med 11:868–876. https://doi.org/10.1002/jgm.1370
3. Backliwal G, Hildinger M, Chenuet S, Wulhfard S, De Jesus M, Wurm FM (2008a) Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Res 36:e96. https://doi.org/10.1093/nar/gkn423
4. Backliwal G, Hildinger M, Hasija V, Wurm FM (2008b) High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol Bioeng 99:721–727. https://doi.org/10.1002/bit.21596
5. Bollin F, Dechavanne V, Chevalet L (2011) Design of experiment in CHO and HEK transient transfection condition optimization. Protein Expr Purif 78:61–68. https://doi.org/10.1016/j.pep.2011.02.008
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