Twin PCRs: a simple and efficient method for directional cloning of PCR products
Author:
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Physiology,Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s11274-011-0670-1.pdf
Reference13 articles.
1. Aslanidis C, de Jong PJ (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res 18:6069–6074
2. Bhat GJ, Lodes MJ, Myler PJ, Stuart KD (1991) A simple method for cloning blunt ended DNA fragments. Nucleic Acids Res 19:398
3. Chen GJ, Qiu NH, Karrer C, Caspers P, Page MPG (2000) Restriction site-free insertion of PCR products directionally into vectors. BioTechniques 28:498–500, 504–505
4. Chen GJ, Qiu NH, Page MPG (2002) Universal restriction site-free cloning method using chimeric primers. BioTechniques 32:516–520
5. Gál J, Schnell R, Szekeres S, Kálmán M (1999) Directional cloning of native PCR products with preformed sticky ends (autosticky PCR). Mol Gen Genet 260:569–573
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