Leakage of recombinant penicillin G acylase from Escherichia coli by adding chloroform under fermentation conditions
Author:
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Physiology,Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s11274-008-9738-y.pdf
Reference11 articles.
1. Ames GF, Prody C, Kustu S (1984) Simple, rapid and quantitative release of periplasmic proteins by chloroform. J Bacteriol 160:1181–1183 Medline
2. Feliu JX, Cubarsi R, Villaverde A (1998) Optimized release of recombinant proteins by ultrasonication of E. coli cells. Biotechnol Bioeng 58:536–540 doi: 10.1002/(SICI)1097-0290(19980605)58:5<536::AID-BIT10>3.0.CO;2-9
3. Gang DM, Shaikh K (1976) Regulation of penicillin acylase in Escherichia coli by cyclic AMP. Biochim Biophys Acta 425:110–114 Medline
4. Jiang YM, Tong WY, Wei DZ (2007) Effects of induction starting time and Ca2+ on expression of active penicillin G acylase in Escherichia coli. Biotechnol Prog 23:1031–1037 doi: 10.1021/bp070074f
5. Kleinig AR, Middelberg APJ (1996) The correlation of cell disruption with homogenizer valve pressure gradient determined by computational fluid dynamics. Chem Eng Sci 51:5103–5110 doi: 10.1016/S0009-2509(96)00354-5
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