A new spike-in-based method for quantitative metabarcoding of soil fungi and bacteria
Author:
Publisher
Springer Science and Business Media LLC
Subject
Microbiology (medical),Microbiology
Link
https://link.springer.com/content/pdf/10.1007/s10123-023-00422-5.pdf
Reference46 articles.
1. Bengtsson-Palme J, Ryberg M, Hartmann M, Branco S, Wang Z, Godhe A, De Wit P, Sánchez-García M, Ebersberger I, de Sousa F et al (2013) Improved software detection and extraction of ITS1 and ITS2 from ribosomal its sequences of fungi and other eukaryotes for analysis of environmental sequencing data. Methods Ecol Evol 4:914–919
2. Bonk F, Popp D, Harms H, Centler F (2018) PCR-based quantification of taxa-specific abundances in microbial communities: quantifying and avoiding common pitfalls. J Microbiol Methods 153:139–147
3. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP (2016) DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods 13:581–583
4. Chen K, Hu Z, Xia Z, Zhao D, Li W, Tyler JK (2016) The overlooked fact: fundamental need for spike-in control for virtually all genome-wide analyses. Mol Cell Biol 36:662–667
5. Davis NM, Proctor DM, Holmes SP, Relman DA, Callahan BJ (2018) Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome 6:226
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